This was one of side projects during my time there because i observed that morphology seems to be determining the intensity and signal dynamics of voltage sensor. For example, the highest intensity is when a cycle rounds up and there are voltage behaviours that only seem to correspond to shape.
Microfabrication of Cell-Scale Structures #
At Harvard’s Center for Nanoscale Systems (CNS), we fabricated microscale patterns designed to match typical cellular dimensions. These precisely engineered substrates provide controlled environments for studying how geometric constraints influence cellular behavior. The nanofabrication facility’s advanced lithography capabilities enabled us to create features with subcellular resolution, establishing a foundation for systematic cell shape analysis.
Voltage Sensor-Based Shape Characterization #
Voltage sensors served as our primary tool for quantifying cellular morphology and shape-dependent properties. By monitoring electrical responses across different geometric configurations, we could correlate cellular shape with functional parameters. This approach provided a quantitative framework for understanding how substrate geometry influences cell behavior, with voltage measurements acting as a sensitive proxy for morphological changes and cellular adaptation to constrained environments.